Summary information and primary citation
- PDB-id
-
8acb;
SNAP-derived features in text and
JSON formats
- Class
- virus like particle
- Method
- cryo-EM (2.6 Å)
- Summary
- Cryoem structure of sweet potato feathery mottle virus
vlp
- Reference
-
Chase O, Javed A, Byrne MJ, Thuenemann EC, Lomonossoff
GP, Ranson NA, Lopez-Moya JJ (2023): "CryoEM and
stability analysis of virus-like particles of potyvirus
and ipomovirus infecting a common host." Commun
Biol, 6, 433. doi: 10.1038/s42003-023-04799-x.
- Abstract
- Sweet potato feathery mottle virus (SPFMV) and Sweet
potato mild mottle virus (SPMMV) are members of the genera
Potyvirus and Ipomovirus, family Potyviridae, sharing
Ipomoea batatas as common host, but transmitted,
respectively, by aphids and whiteflies. Virions of family
members consist of flexuous rods with multiple copies of a
single coat protein (CP) surrounding the RNA genome. Here
we report the generation of virus-like particles (VLPs) by
transient expression of the CPs of SPFMV and SPMMV in the
presence of a replicating RNA in Nicotiana benthamiana.
Analysis of the purified VLPs by cryo-electron microscopy,
gave structures with resolutions of 2.6 and 3.0 Å,
respectively, showing a similar left-handed helical
arrangement of 8.8 CP subunits per turn with the C-terminus
at the inner surface and a binding pocket for the
encapsidated ssRNA. Despite their similar architecture,
thermal stability studies reveal that SPMMV VLPs are more
stable than those of SPFMV.
